Chemistry Practical 12
Certainly! Here's a sample guide for Class 12 Chemistry Practical (as per general CBSE/ISC guidelines). Let’s focus on Chemistry Practical No. 12, though practical numbering may vary depending on your school board or manual.
🧪 Chemistry Practical 12: Qualitative Analysis – Identification of Functional Groups in Organic Compounds
🔍 Objective:
To identify the functional group present in the given organic compound.
🧬 Principle:
Organic compounds are classified based on the functional groups they contain such as:
- 
Alcohols (-OH)
 - 
Aldehydes (-CHO)
 - 
Ketones (C=O)
 - 
Carboxylic acids (-COOH)
 - 
Amines (-NH₂)
 - 
Phenols (-OH attached to aromatic ring)
 
These groups respond differently to specific reagents in qualitative tests.
🧫 Apparatus and Chemicals Required:
| Apparatus | Chemicals | 
|---|---|
| Test tubes | Fehling's solution A & B | 
| Test tube holder | Tollens' reagent | 
| Dropper | Sodium bicarbonate solution | 
| Bunsen burner | Bromine water | 
| Beaker | Neutral FeCl₃ solution | 
| Pipette | Alcohol, aldehyde, ketone samples | 
🧪 Tests and Observations:
| Test | Functional Group | Observation | Inference | 
|---|---|---|---|
| Sodium bicarbonate test | -COOH (acid) | Effervescence due to CO₂ | Carboxylic acid | 
| Fehling’s test | -CHO (aldehyde) | Red precipitate | Aldehyde present | 
| Tollens’ test | -CHO (aldehyde) | Silver mirror | Aldehyde present | 
| 2,4-DNP test | -C=O (aldehyde/ketone) | Yellow/orange ppt | Carbonyl group | 
| Ferric chloride test | -OH (phenol) | Violet/green color | Phenol | 
| Lucas test | Alcohol | Cloudy solution (tertiary fastest) | Alcohol group | 
✍️ Procedure (Example for Carboxylic Acid):
- 
Take a small amount of the compound in a test tube.
 - 
Add a few drops of sodium bicarbonate solution.
 - 
Observe for effervescence (bubbling due to CO₂).
 - 
Confirm the evolution of CO₂ by passing gas through limewater.
 
🧾 Result:
The functional group present in the given compound is: [Your Observed Result]
🧠 Precautions:
- 
Use minimum quantity of chemicals.
 - 
Perform tests in a well-ventilated area.
 - 
Wear gloves and safety glasses.
 - 
Do not inhale vapors of organic compounds.
 
If you let me know your board (CBSE, ISC, State Board) or specific practical title, I can tailor it exactly to your syllabus.
Would you like the full PDF notes or viva questions with answers for this practical?
